【摘要】 目的观察miR-23a在扩张型心肌病(DCM)外周血中的表达差异,评估其临床意义。方法收集26例DCM患者作为病例组,收集30例健康者作为对照组。比较两组的临床资料和心脏超声结果,检测脑尿钠肽(BNP)等生化指标,用Real-time PCR检测和比较两组的miR-23a表达水平的差异,并通过ROC曲线分析评估miR-23a对DCM患者的识别能力。结果 DCM组的BNP(605.00±231.71,26.29±17.88,t=218.61,P<0.05)、左室舒张末直径(LVEDD)高于对照组(64.73±5.74,45.58±2.17,t=6.09,P<0.05),左室射血分数(LVEF)低于对照组(35.33±4.31,58.72±3.35,t=-13.54,P<0.05)。miR-23a在DCM组中的表达量高于对照组(3.56±2.56,1.18±0.86,t=2.85,P<0.01),差异有统计学意义。ROC曲线分析发现,miR-23a对DCM患者具有一定的临床识别能力[AUC=0.781,95%CI(0.651,0.911),P<0.01]。结论 miR-23a在DCM患者外周血中的表达明显升高,miR-23a可能在DCM患者中具有一定的临床诊断价值。更多还原

【Abstract】 Objective To observe the expression of miR-23 a in dilated cardiomyopathy(DCM) of the peripheral blood and assess its clinical significance.Methods Twenty-six patients with dilated cardiomyopathy and 30 healthy controls were enrolled.The clinical data of the two groups,cardiac ultrasound,plasma level of brain natriuretic peptide(BNP) and other biochemical indicators were collected respectively.Real-time polymerase chain reaction(PCR) was used to detect and compare two groups’ s miR-23 a expression level differences,ROC curve analysis was used to assess miR-23 a recognition in patients with DCM.Results BNP(605.00±231.71,26.29±17.88,t=218.61,P<0.05) and left ventricular end-diastdic diameter(LVEDD)(64.73±5.74,45.58±2.17,t=6.09,P<0.05) in DCM group were higher than those in control group,left ventricular ejection fraction(LVEF) lower than that in control group(35.33±4.31,58.72±3.35,t=- 13.54,P<0.05).miR-23 a expression in the DCM group was higher than that in control group(33.56±2.56,1.18±0.86,t=2.85,P<0.01),the difference was statistically significant.ROC curve analysis demonstrated that miR-23 a had certain clinical recognition ability in patients with DCM [AUC=0.781,95% CI(0.651,0.911),P<0.01].Conclusion The expression of miR-23 a elevated in the peripheral blood of patients with DCM,miR-23 a may have certain clinical diagnostic value in patients with DCM.更多还原