【摘要】 目的研究紫玉盘内生真菌节菱孢Arthrinium sp.A092发酵菌丝体的化学成分。方法采用硅胶柱、凝胶柱、HPLC等色谱技术进行分离纯化,根据理化性质和波谱数据进行化合物结构鉴定;以肝癌细胞Hep G-2、乳腺癌细胞MCF-7、大细胞肺癌细胞NCI-H460和神经胶质瘤细胞SF-268为供试细胞株,采用SRB法对化合物进行细胞毒活性测试。结果从节菱孢菌丝体中分离得到10个化合物,分别鉴定为3,4,5-trimethyl-6-methoxy-8-hydroxyisocoumarin(1)、decarboxycitrinone(2)、4-hydroxy-17R-methylincisterol(3)、3-甲氧基-4-羟基苯甲酸(4)、邻苯二甲酸二丁酯(5)、flemingipanic acid(6)、吲哚-3-羧酸(7)、过氧化麦角甾醇(8)、对羟基苯甲酸(9)、4-羟基苯甲醛(10);化合物3对SF-268和MCF-7的IC50值分别为63.8和57.2?mol/L,化合物8对SF-268、MCF-7和NCI-H460的IC50值分别为50.6、32.3、39.0?mol/L。结论化合物1为新化合物,命名为节菱孢异香豆素A,化合物3~5、7~10均为首次从该属真菌中分离得到;化合物3和8具有一定的细胞毒活性。更多还原

【Abstract】 Objective To study the chemical constituents in fermented mycelium of endophytic fungus Arthrinium sp.A092 from Uvaria microcarpa.Methods The compounds were isolated and purified by chromatography on silica gel,Sephadex-LH20 columns,HPLC,and so on.Their structures were identified on the basis of physicochemical properties and spectroscopic data.All compounds were tested for their cytotoxic activities against four tumor cell lines Hep G-2,MCF-7,NCI-H460,and SF-268.Results Ten compounds were isolated from the mycelial extract and identified as 3,4,5-trimethyl-6-methoxy-8-hydroxyisocoumarin(1),decarboxycitrinone(2),4-hydroxy-17R-methylincisterol(3),4-hydroxy-3-methoxybenzoic acid(4),dibutyl phthalate(5),flemingipanic acid(6),indole-3-carboxy acid(7),ergosterol peroxide(8),p-hydroxybenzoic acid(9),and 4-hydroxybenzal-dehyde(10).Compounds 3 exhibited the inhibitory activity against SF-268 and MCF-7 with IC50 values of 63.8 and 57.2 ?mol/L,respectively.Compounds 8 exhibited the inhibitory activity against SF-268,MCF-7,and NCI-H460 with IC50 values of 50.6,32.3,and 39.0 ?mol/L,respectively.Conclusion Compound 1 is a new compound named arthrinisocoumarin A.Compounds 3—5 and 7—10 are isolated from the fungus of Arthrinium sp.for the first time.Compounds 3 and 8 show the moderated cytotoxic activities against SF-268,MCF-7,and NCI-H460.更多还原