【摘要】 目的建立强力天麻杜仲胶囊(天麻、杜仲、独活等)的HPLC特征图谱。方法强力天麻杜仲胶囊70%乙醇提取液的分析采用Cortecstm C18色谱柱(4.6 mm×150 mm,2.7μm);流动相乙腈-0.1%磷酸水溶液,梯度洗脱;体积流量0.5 m L/min;柱温40℃;检测波长220 nm。结果该药物碱性、酸性乙酸乙酯部位的HPLC特征图谱分别有28个和18个共有峰,鉴定出3个(天麻素、紫花前胡苷、7-羟基香豆素)和4个(没食子酸、原儿茶酸、绿原酸、紫云英苷)色谱峰,10批样品特征图谱相似度为0.85~0.99。结论该方法准确可靠,可用于强力天麻杜仲胶囊的质量控制。更多还原

【Abstract】 AIM To establish the characteristic HPLC chromatograms of Qiangli Tianmaduzhong Capsules( Gastrodiae Rhizoma,Eucommiae Cortex,Angelicae Pubescentis Radix,etc.). METHODS The analysis of Qiangli Tianmaduzhong Capsules 70% ethanolic extract was carried out on a Cortecstm C18column( 4. 6 mm ×150 mm,2. 7 μm),mobile phase was acetonitrile-0. 1% phosphoric acid aqueous solution in a gradient elution manner,flow rate was 0. 5 m L / min,column temperature was maintained at 40 ℃,and detection wavelength was set at 220 nm. RESULTS There were twenty-eight and eighteen common peaks in the characteristic HPLC chromatograms of alkaline ethyl acetate and acid ethyl acetate extracts of this drug,then three( gastrodin,nodakenin,7-hydroxycoumarinand) and four( gallic acid,protocatechuate,chlorogenic acid,astragalin) chromatographic peaks were identified,respectively. The similarities of characteristic chromatograms of ten batches of samples were0. 85- 0. 99. CONCLUSION This accurate and reliable method can be used for the quality control of Qiangli Tianmaduzhong Capsules.更多还原