【摘要】 目的建立超高效液相色谱-串联质谱(UHPLC-MS/MS)法同时测定蒲地蓝消炎片(蒲公英、黄芩、苦地丁和板蓝根)中腺苷、表告依春、咖啡酸、绿原酸、阿魏酸、紫堇灵、乙酰紫堇灵、芹菜素、黄芩素和汉黄芩素的含有量。方法分析采用Eclipse Plus C18RRHD色谱柱(1.8μm,2.1 mm×50 mm);流动相为乙腈-0.2%乙酸水溶液,梯度洗脱;体积流量为0.3 m L/min。结果 10种成分分别在0.000 050 0~1.00、0.010 0~5.00、0.015 0~10.0、0.030 0~20.0、0.100~100、0.000 300~10.0、0.003 00~10.0、0.000 600~20.0、0.200~20.0、0.000 500~5.00 mg/L范围内线性关系良好,平均加样回收率在91.4%~105.0%之间,RSD均小于2.0%。结论该方法快速、简便、灵敏,适用于蒲地蓝消炎片的质量控制。更多还原

【Abstract】 AIM To establish an ultra performance liquid chromatography with tandem mass spectrometry(UHPLC-MS/MS) method for the simultaneous determination of adenosine,epigoitrin,caffeic acid,chlorogenic acid,ferulic acid,corynoline,acetylcorynoline,apiginin,baicalein and wogonin in Pudilan Xiaoyan Tablets(Taraxaci Herba,Scutellariae Radix,Corydalis Bungeanae Herba and Isatidis Radix).METHODS The analysis was performed on an Eclipse Plus C18 RRHD column(1.8 μm,2.1 mm × 50 mm),with a mobile phase comprising of acetonitrile-0.2% acetic acid aqueous solution in a gradient elution manner,at a flow rate of 0.3 m L/min.RESULTS Ten constituents showed good linear relationships within the ranges of 0.000 050 0-1.00 mg/L,0.010 0-5.00 mg/L,0.015 0-10.0 mg/L,0.030 0-20.0 mg/L,0.100-100 mg/L,0.000 300-10.0 mg/L,0.003 00-10.0 mg/L,0.000 600-20.0 mg/L,0.200-20.0 mg/L and 0.000 500-5.00 mg/L,respectively,whose average recoveries were 91.4%-105.0% with the RSDs of less than 2.0%.CONCLUSION This rapid,simple and sensitive method suits the quality control of Pudilan Xiaoyan Tablets.更多还原