目的:探讨含非甲基化Cp G基序的寡脱氧核苷酸(Cp G oligodeoxyribonucleotides 7909,Cp G ODN7909)与Toll样受体(Toll-like receptor,TLR)9结合后是否影响人肺癌A549和H520细胞对多西他赛(doctaxel,DOC)的化疗敏感性及相关机制。方法:设计并合成特异性干扰siRNA序列,转染细胞,Western blot法检测TLR9 siRNA的沉默效果,CCK-8法检测细胞活力。设空白对照组、阴性对照组和TLR9 siRNA干扰组,分别接受Cp G ODN7909和多西他赛单独或联合治疗,流式细胞术检测细胞周期及凋亡,Western blot法检测P38及Bax蛋白表达。结果:在2种细胞中,Cp G ODN7909单独处理后,细胞活力和凋亡率都无明显变化,G2/M期细胞比例P38和Bax蛋白的表达明显升高(P<0.01);Cp G ODN7909处理TLR9 siRNA干扰细胞各指标均无明显变化。多西他赛单独处理后3组细胞的细胞活力明显下降,G2/M期细胞比例、凋亡率和Bax蛋白表达都明显升高(P<0.01),P38表达无明显变化。与多...
【英文摘要】
AIM: To probe whether Cp G oligodeoxyribonucleotides 7909( Cp G ODN7909) combined with Toll like receptor( TLR) 9 affected the chemotherapeutic sensitivity of doctaxel( DOC) in human lung cancer A549 and H520 cell lines. METHODS: Sequences of TLR9 siRNAs were designed. A549 and H520 cells were transfected with TLR9 siRNA by lipofectamine. The expression of TLR9 was detected by Western blot. The cell activity was measured by CCK-8 assay. The experiments were divided into blank control group,control siRNA gro...
【基金】
上海市卫计委青年课题(No.2013-156)
【更新日期】
2016-11-01
【分类号】
R734.2
【正文快照】
cing the inhibitory effect and apoptosis of DOC on the cell activity in vitro,arresting the cells at G2/M phase of the cells.Toll样受体(Toll-like receptors,TLRs)是存在于哺乳动物且与Toll蛋白同源的分子,是表达于多种免疫细胞的模式识别受体(pattern recognition
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