AIM: To observe the influence of polysaccharides extracted from Retinervus luffae fructus( RLF)on the differentiation of 3T3-L1 pre-adipocytes and to investigate its mechanism. METHODS: DEAE-cellulose column was used to isolate and purify RLF. The effect of RLF polysaccharides on 3T3-L1 pre-adipocyte differentiation was determined by oil red O staining. The effect of RLF on the mRNA expression of differentiation-related factors C / EBPβ,PPARγ and C /EBPα was detected by RT-q PCR. RESULTS: Two components of ...