【摘要】 目的研究血管生成素1和2(Ang-1、Ang-2)在糖尿病视网膜病变(DR)患者血清中的表达情况及其与DR病变程度的相关性。方法共纳入2型糖尿病(T2DM)患者160例,包括无DR患者63例(NDR组)、非增生型DR者51例(NPDR组)和增生型糖尿病视网膜病变患者46例(PDR组)。用酶联免疫吸附测定法(ELISA)检测Ang-1、Ang-2及内皮细胞特异性受体酪氨酸激酶(Tie-2)在糖尿病视网膜病变患者血清的表达水平。分析Ang-1、Ang-2与DR患者临床病理参数与比值之间的关系。结果在NPDR组血清Ang-2较NDR组明显升高,而且PDR患者的血清Ang-2较NPDR患者升高更明显;NPDR组血清Ang-1较PDR组、NDR组略有升高,但各组患者的血清Ang-1差异无统计学意义(P>0.05)。PDR组和NPDR组间患者的血清Ang-2水平和Ang-1/Ang-2比值比较,差异有统计学意义(P均<0.05)。NDR组、NPDR组和PDR组Ang-1/Ang-2比值依次为(3.57±1.32)、(2.36±0.64)和(1.70±0.54),差异有统计学意义(P<0.001)。各组患者Tie-2差异无统计学意义(P>0.05)。结论 Ang-1/Ang-2比值与DR相关,随病情加重,水平呈下降趋势,该比值可用于辅助诊断DR。更多还原

【Abstract】 Objective To explore the expression levels of angiopoietin 1(Ang-1) and angiopoietin 2(Ang-2) in the serum of patients with diabetic retinopathy(DR) and their relationship with condition.Methods A total of 160 patients with type 2 diabetes mellitus(T2DM) were involved in this study,including of 63 patients without DR(NDR group),51 patients with non proliferative diabetic retinopathy(NPDR group) and 46 patients with proliferative diabetic retinopathy(PDR group).The expression of Ang-1,Ang-2 and endothelial-specific receptor tyrosine kinase(Tie-2) in the sera from all of the investigated individuals were detected by ELISA method.Meanwhile,we analyzed the relationship between the clinical pathological parameters and the ratio of Ang-1/Ang-2 in these patientsby statistics softwares.Results Compared with NDR group,the level of Ang-2 in NPDR group was increased,but was elevated significantly in PDR group(all P<0.01).The level of Ang-1 in NPDR group was higer than that in Group PDR and Group NDR,but there was no significant difference among them(all P>0.05).The levels of Ang-2 and the ratio of Ang-1/Ang-2 were significantly different between PDR group and NPDR group(P<0.05).The ratio of Ang-1/Ang-2 were 3.57±1.32,2.36±0.64,1.70±0.54 in NDR,NPDR,PDR group,respectively,and significant difference was found among them(P<0.001),but there was no difference in the levelof Tie-2 among these three groups(P>0.05).Conclusions The ratio of Ang-1/Ang-2 is related to the occurrence of DR,which decreases with the DR development and the ratio has accessory diagnostice value for DR.更多还原