【摘要】 目的:优选枸杞多糖的最佳提取工艺,通过建立D-半乳糖致衰老小鼠模型,研究枸杞多糖的抗衰老作用。方法:采用正交设计法对提取工艺进行研究;应用D-半乳糖连续注射,造成实验性衰老小鼠模型,通过测定血液丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-PX)和过氧化氢酶(CAT)的含量及超氧化物歧化酶(SOD)的活力和皮肤中SOD、MDA及羟脯氨酸(Hyp)的水平来检测枸杞多糖抗衰老的能力。结果:通过正交设计确定的枸杞多糖的最佳提取方案为:料液比为1∶30,提取2次,1次90 min,功率为100 k Hz。通过研究可见,枸杞多糖可提高小鼠血液中SOD、CAT、GSH-px水平,降低MDA值;可提高小鼠皮肤SOD活力,降低皮肤MDA含量,提高Hyp含量。结论:本研究建立的提取方法简单、易行,枸杞多糖的提出率高。枸杞多糖具有抗D-半乳糖所致小鼠衰老的作用。更多还原

【Abstract】 OBJECTIVE: To optimize the optimum extraction technology of lycium barbarum polysaccharide and establish senile mice model by D-galactose,so as to research the anti-aging effects of lycium barbarum polysaccharide.METHODS: Orthogonal design method was adopted to research the extraction technology. The senile mice model was established by injection of D-galactose. The anti-aging capacibility of lycium barbarum polysaccharide were determined by the detection of malonaldehyde( MDA),glutathione peroxidase( GSH-PX),the content of catalase( CAT),activity of superoxide dismutase( SOD) and level of SOD,MDA and hydroxyproline( Hyp) in the skin. RESULTS: The optimum extraction technology scheme by orthogonal design method were: the ratio of solid to liquid was 1 ∶ 30,2 times of extraction,90 min for once,the power was 100 k Hz. Lycium barbarum polysaccharide could increase the level of SOD,CAT and GSH-px in rats,decrease MDA,improve the SOD activity,decrease MDA and increase the content of Hyp in the skin of mice. CONCLUSIONS: The extraction method established by this research is simple and feasible,with high extraction rate of lycium barbarum polysaccharide. Meanwhile,lycium barbarum polysaccharide has significant effects on the senile mice model induced by injection of D-galactose.更多还原