【摘要】 目的建立高效液相色谱（HPLC）法测定苦参碱醇质体温敏凝胶中苦参碱含量。方法采用YMC-Pack NH2柱（4.6 mm×150 mm,5μm）,以乙腈-乙醇（9∶1）为流动相,流速为0.8 m L·min^-1,柱温（25±2）℃,检测波长为215 nm。结果苦参碱在1.00¹00.00μg·m L^-1范围内峰面积与浓度呈良好的线性关系（R2=0.999 2）,低、中、高浓度苦参碱平均加样回收率分别为98.16%,98.15%,98.00%,RSD为分别为0.30%,0.69%,0.71%。结论该方法操作简单易行,结果灵敏准确,专属性强,精密度高,稳定性好,重复性好,能够用于苦参碱醇质体温敏凝胶中苦参碱的含量测定。更多还原

【Abstract】 Objective To establish an HPLC method for determination of matrine in matrine ethosome thermosensitive gels. Methods Matrine was detected in matrine ethosome thermosensitive gels by using a YMC-Pack NH2column（ 4.6 mm×150 mm,5 μm）,with the mobile phase containing acetonitrile-ethanol（ 9∶1）. The flow rate was 0.8 m L·min^-1,the column temperature（ 25 ± 2） ℃ and the UV detection wavelength 215 nm. Results The peak area correlated with matrine concentration within the range of 1.00^-100.00 μg·m L^-1linearly,and the average correlation coefficient was 0.999 2.The average recoveries rate of low,medium and high concentration of matrine were 98.16%,98.15%,98.00%,respectively,and the RSD were0.30%,0. 69%,0. 71%,respectively. Conclusion The method is simple,sensitive and accurate. With the advantages of specificity,high precision,good stability and repeatability,the HPLC method can be used for the determination of matrine in matrine ethosome thermosensitive gels.更多还原