【摘要】 目的观察帕瑞昔布钠对老年抑郁大鼠手术后认知能力的影响。方法老年雄性SD大鼠36只,选取6只作为正常对照组,剩余30只建立抑郁模型,分为手术治疗组和帕瑞昔布钠组,各15只。帕瑞昔布钠组分别于手术前1 h,手术后8 h,第2,3天腹腔注射帕瑞昔布钠5 mg獉kg-1;手术治疗组腹腔注射0.9%氯化钠注射液。采用旷场、糖水消耗实验评价抑郁状态,Morris水迷宫评价学习认知能力,酶联免疫吸附法检测大鼠海马白细胞介素(IL)-1β、IL-6表达情况。结果帕瑞昔布钠组和手术治疗组逃避潜伏期分别为(39.98±8.93),(47.15±10.87)s(P<0.05);空间探索时间分别为(24.17±8.52),(20.96±9.58)s,(P<0.05);海马IL-1β表达分别为(350.73±28.04),(423.35±26.81)ng·L-1(P<0.05);IL-6表达分别为(72.86±6.82),(79.26±4.19)ng·L-1(P<0.05);帕瑞昔布钠组水平、垂直评分均明显高于手术治疗组(均P<0.05),糖水消耗、糖水偏爱均比手术治疗组明显增加,纯水消耗显著减少(P<0.05)。结论帕瑞昔布钠能改善老年抑郁大鼠短期手术后认知能力,其机制可能与降低海马区炎症因子IL-1β、IL-6的表达有关。更多还原

【Abstract】 Objective To investigate the effect of parecoxib sodium on postoperative cognitive dysfunction( POCD) in elderly depression rats. Methods The total of 36 elderly male Sprague Dawley( SD) rats were divided into 3 groups: normal control group( n = 6); surgical group( Sur group,n = 15); paricoxib sodium group( Pare group,n = 15).Depression models were established in rats except those in the normal control group.The 5 mg·kg-1parecoxib sodium was i.p.injected in the Pare group1 h prior to,8 h,2 d and 3 d post operation,respectively.And 0.9% sodium chloride injection was i.p.injected into the Sur group.Behavior and cognition of all animals were evaluated by open-field,sucrose consumption and Morris water maze tests.IL-6 and IL-1β expression were detected by Elisa. Results The escape latency was( 39.98±8.93) s in Pare group vs( 47.15±10.87) s in Sur group( P<0.05); and the space exploration was( 24. 17 ± 8. 52) s vs( 20. 96 ± 9. 58) s( P < 0. 05). IL-6 expression was( 72.86±6.82) in Pare group vs( 79.26±4.19) ng·L-1in Sur group,IL-1β expression was( 350.73±28.04) vs( 423.35±26.81)ng·L-1( P<0.05).The horizontal and vertical scores were significantly higher in Pare group than those in the Sur group( P<0.05).The sucrose consumption and sucrose preference were higher and the water consumption was lower in Pare group than those in the Sur group( P < 0. 05). Conclusion Parecoxib sodium may improve postoperative cognition of elderly rats with depression,which being associated with decrease of IL-6 and IL-1β expression in hippocampus.更多还原