【摘要】 目的探讨Livin、Caspase-8在人骨肉瘤组织中的表达及临床意义。方法选择2008年3月~2015年3月于唐山市人民医院就诊的骨肉瘤患者64例为观察组,取其骨肉瘤组织作为生物样本;选择同期就诊的非骨肉瘤患者64例为对照组,取其正常骨痂组织作为生物样本。按照骨肉瘤诊治规范对相关生物样本进行分析,分析观察组所有样本的肿瘤位置、肿瘤大小、病理分型、Enncking分期等;检测两组样本中Livin和Caspase-8的表达水平;分析其与肿瘤生物学行为的相关性。结果 Livin在观察组骨痂组织中阳性表达率显著高于对照组(P<0.05),Caspase-8在观察组骨痂组织的阳性表达率显著低于对照组(P<0.05);性别、年龄、肿瘤位置和病理分型与Livin和Caspase-8的表达水平无关(P>0.05),而肿瘤的大小、Enncking分期和患者最终生存时间与Livin和Caspase-8的表达相关(P<0.05或P<0.01);Livin蛋白表达和Caspase-8蛋白表达呈负相关(r=-0.392,P<0.05)。结论骨肉瘤组织中Livin的表达量显著升高,而Caspase-8的表达量显著降低,且Livin和Caspase-8的表达与骨肉瘤的肿瘤大小、Enncking分期及患者最终生存时间有关。更多还原

【Abstract】 Objective To investigate the expression and clinical significance of Livin and Caspase-8 in human osteosarcoma. Methods The osteosarcoma cancer tissues of 64 patients with osteosarcoma cancer who were examined and treated in Tangshan People’s Hospital from March 2008 to March 2015 were collected as the observation group. Another 64 patients with non-osteosarcoma cancer were selected as the control group. The relevant biological samples were analyzed in accordance with the standard of diagnosis and treatment of osteosarcoma cancer. The tumor location, tumor size, pathological type, Enncking stage and so on of the observation group were analyzed and observed. The expression level of Caspase-8 and Livin was detected in the two groups and its correlation with tumor biological behavior was analyzed. Results The positive expression rate of Livin in the observation group was significantly higher than that in the control group(P < 0.05). The positive expression rate of Caspase-8 in the observation group was significantly lower than that in the control group(P < 0.05). Sex, age, tumor location and pathological type were independent with the expression levels of Livin and Caspase-8(P > 0.05), however, tumor size, Enncking stage and the final survival time were correlated with the expression of Caspase-8 and Livin protein(P < 0.05 or P < 0.01). The expression of Livin protein was negatively correlated with the expression of Caspase-8 protein(r =-0.392, P < 0.05). Conclusion In osteosarcoma tissue, the expression of Livin is increased significantly and caspase-8 was significantly reduced. The expression levelof Livin and Caspase-8 is related to the tumor size,Enncking stage and the final survival time of osteosarcoma.更多还原