【摘要】 目的:筛选具有体外抑制脂肪酸合酶活性的天然产物,研究中药砂仁、虎杖及桂枝萃取液对MCF-7乳腺癌细胞的抑制作用。方法:将细胞株按照不同中药萃取液处理方式分为实验组与对照组,实验组中分为虎杖组、白藜芦醇组、虎杖苷组、砂仁组以及桂枝组;对照组以无血清RMPI1640培养液代替药物。采用CCK-8法观测不同浓度的砂仁、虎杖及桂枝以及其主要成分白藜芦醇和肉桂酸,作用24 h后对MCF-7乳腺癌细胞增殖的影响。结果:砂仁、虎杖及桂枝中60%乙醇提取物IC50分别为24.86μg/ml、153.67μg/ml及178μg/ml;白藜芦醇的抑癌活性强于虎杖,IC50为61.75μg/ml;桂枝的主要成分肉桂酸在低浓度时抑癌活性强。结论:砂仁、虎杖及桂枝60%乙醇提取物均能显著抑制MCF-7乳腺癌细胞生长,且呈现出浓度依赖性,其中砂仁活性最强,效果最显著。更多还原

【Abstract】 Objective: To screen fatty acid synthase（FAS） inhibitors from natural products and study their inhibitory effects on the proliferation of MCF-7 breast cancer cells.Methods: CCK-8 method was used to detect the inhibitory effects of Fructus Amomi,Polygonum cuspidatum Sieb,Cinnamomi Ramulus and their main compounds,such as polydatin,resveratrol and cinnamic acid on the proliferation of MCF-7 breast cancer cells for 24 h.Results: The results showed that the IC₅₀ values of the 60% ethanol extracts of Fructus Amomi,Polygonum cuspidatum Sieb,and Cinnamomi Ramulus were 24.86μg/ml,153.67 μg/ml and 178 μg/ml respectively.The IC₅₀ value of Resveratrol was 61.75 μg/ml.The inhibitory effect of Resveratrol was better than that of Polygonum cuspidatum Sieb.Cinnamic acid,the main component of Cinnamomi Ramulus had better inhibitory activity at lower concentration.Conclusion: The 60% ethanol extracts of Fructus Amomi,Polygonum cuspidatum Sieb,and Cinnamomi Ramulus all showed inhibitory effects on the proliferation of MCF-7 breast cancer cells in a dose-dependent manner.Among them,Fructus Amomi had the best inhibitory activity.更多还原