【摘要】 目的探讨P选择素糖蛋白配体-1(P-selectin glycoprotein ligand-1,PSGL-1)Met62Ile(M62I)基因多态性在中国广东地区汉族人群中的分布特点及其与冠心病(coronary heart disease,CHD)的相关性。方法应用聚合酶链反应-限制性片段长度多态性(polymerase chain reaction restrictionfragment length polymorphism,PCR-RFLP)分析方法检测200名该地区冠心病患者和200名汉族健康人群PSGL-1 M62I基因型,计算并比较各组基因型频率及等位基因频率。结果 200名冠心病组中P选择素PSGL-1 M62I基因型以Met/Met(M/M)型发生频率最高(73.0%),Met/Ile(M/I)型次之(27.0%),未检测到Ile/Ile(I/I)型;M、I各型等位基因频率分别为86.5%、13.5%,该位点基因多态性分布在男女间无显著性差异(P>0.05)。200名正常对照组中P选择素PSGL-1 M62I基因型以M/M型发生频率最高(69.0%),M/I型次之(31.0%)未检测到I/I型;M、I各型等位基因频率分别为84.5%、15.5%,该位点基因多态性分布在男女间无显著性差异(P>0.05)。两组人群间各基因型及等位基因分布无显著性差异(P>0.05)。结论中国广东地区冠心病患者和健康人群PSGL-1 M62I基因多态性在男女之间均无较大的差异。PSGL-1 M62I位点可能不是冠心病的易感基因。更多还原

【Abstract】 Objective To investigate the different age groups distribution of P-selectin glycoprotein ligand- 1(PSGL- 1) Met62Ile(M/I) gene polymorphism in the Chinese Han population and the relationship between the polymorphism and coronary heart disease(CHD). Methods Polymerase chain reaction restriction fragment length polymorphism(PCR- RFLP) analysis was used to detect the genotype and allele frequency of PSGL- 1 M62 I in 200 patients with CHD and then the genotype and allele frequencies were compared to a control population of 200 healthy, Han Chinese. Results In the 200 CHD patients of Guangdong area, the highest frequency of PSGL-1 M62 I genotype is M/M(73.0%), followed by M/I(27.0%). The I/I genotype was not detected; M, I allele frequencies were 86.5% and 13.5%. These gene polymorphisms had no significant difference between males and females(P>0.05). In the healthy control population, the highest frequency of PSGL-1 M62 I genotype is M/M(69.0%), followed by M/I(31.0%). The I/I genotype was also not detected. The allele frequencies of M and I were 86.75% and 13.25%. These gene polymorphisms had no significant difference between males and females(P>0.05). There were no significant differences between CHDgroup and normal group(P>0.05). Conclusion Between the CHD patients and healthy Han Chinese population of the Guangdong area in China, there were no significant differences in PSGL- 1 M62 I gene polymorphism. The PSGL-1 M62 I gene may not be the susceptibility gene of CHD.更多还原