【摘要】 建立大鼠血浆中朝藿定A、B、C及淫羊藿苷的LC-MS/MS法,并应用于大鼠肌肉注射喘可治注射液后血浆中4种黄酮苷浓度的测定及喘可治注射液的药物动力学研究。采用的色谱条件为Agilent Eclipse XDB-C₁₈（150 mm×2.1 mm,5.0μm）色谱柱,乙腈-0.1%甲酸水溶液（35∶65）为流动相,流速0.22 mL·min^-1,柱温40℃;质谱条件为电喷雾多反应离子监测（MRM）负离子检测模式,血浆样品采用固相萃取法处理。结果显示,朝藿定A、B、C及淫羊藿苷在1¹ 000 ng·m L^-1浓度内具有良好的线性关系;日内精密度RSD<5.99%,日间精密度RSD<10.16%;各成分相对回收率介于88.1%¹01.1%,RSD<7.9%,绝对回收率介于72.0%⁸6.6%,RSD<6.3%。研究表明,该分析方法专属、灵敏、快速,适用于血浆中淫羊藿黄酮苷浓度的测定。更多还原

【Abstract】 A quantitative method for epimedin A, B, C and icariin in rat plasma was established using LC-MS/MS after intermuscular administration of Chuankezhi injection to rat. Chromatographic separation was performed on an Agilent Eclipse XDB-C₁₈ column（150 mm × 2.1 mm, 5.0 μm） at 40 ℃. Mobile phase consisted of acetonitrile-0.1% formic acid in water（35∶65）, and the flow rate was 0.22 m L·min^-1. The LC effluent was detected and analyzed using an ESI-triple quadrupole tandem mass spectrometer under the multiple reaction monitoring（MRM） in the negative ion mode. The plasma samples were treated with solid phase extraction prior to LC-MS/MS analysis. As a result, all of the four analytes displayed a good linearity over the concentration of 1-1 000 ng·m L^-1. The RSDs of intra-day and inter-day assays were less than 5.99% and 10.16%, respectively. The relative recovery of each analyte was between 88.1%-101.1% with RSD < 7.9% and the absolute recovery was between 72.0%-86.6%（RSD < 6.3%）. In conclusion, the established method shows good specificity, sensitivity and efficiency for quantifying the four flavonoid glycosides contained in rat plasma.更多还原