To protect the wild Thesium chinense Turcz resources,through plant tissue culture technique,a large number of Thesium chinense Turcz seedlings were successfully cultivated. The results showed that the optimal explant material was stem and culture medium was MS + 2,4-D 1. 0 mg/L on callus induction. Best culture medium of callus sub-cultivation was MS + 2,4-D 0. 5 mg/L. Bud induction in MS + 6-BA 2. 0 mg/L + NAA 0. 25 mg/L and MS + 6-BA 2. 0 mg/L + NAA 0. 125 mg/L can obtain better effect. Seedlings sub-cult...