【摘要】 目的观察免疫球蛋白A肾病(IgAN)肾组织中的IgA与乙型肝炎病毒(HBV)抗原(HBVAg)及血清IgA之间的关系。方法用免疫荧光双套染技术配合激光共聚焦显微镜观察IgA在66例不同类型IgAN肾小球中的沉积量;免疫组织化学二步法检测66例IgAN肾组织HBV表面抗原(HBs Ag)、HBV核心抗原(HBc Ag),对比研究IgA沉积量与HBVAg感染及血清IgA的关系。结果免疫荧光双套染法显示IgA主要在肾小球系膜区、少量在毛细血管袢呈颗粒状沉积;HBVAg阳性的肾组织IgA沉积量明显高于HBVAg阴性组(P<0.05)与对照组(P<0.05),且与肾脏病变程度有关。IgA肾病肾组织中IgA沉积量与血清IgA差异无统计学意义。结论 HBVAg阳性的IgA肾病肾组织IgA沉积量与IgA肾病发展存在一定的关系,血清IgA浓度的高低可能不是IgA肾病发生的决定性因素。更多还原

【Abstract】 Objective To investigate the correlation between imumnoglobulin A(IgA) of IgA nephropathy(IgAN) in kidney tissues and hepatitis B virus antigens(HBVAg), and serum IgA, respectively. Methods The IgA precipitation in glomerular of 66 different types of IgAN was determined by immunofluorescence double staining and laser confocal microscopy. The status of HBs Ag and HBc Ag were determined by two-step immunohistochemistry in 66 cases of IgAN tissues. The correlation between IgA precipitation and HBVAg infection and serum IgA was examined and compared, respectively. Results Immunofluorescence double staining showed that IgA was mainly in the glomerular mesangial area. A small amount of IgA granular deposition was found in the capillary loop. The precipita-tion of IgA in positive HBVAg kidney tissues was significantly higher than that in the negative HBVAg group( P<0.05)and control group(P<0.05), which was correlated with the degree of renal lesions. There was no statistically significant difference between IgA precipitation and serum IgA in IgAN tissues. Conclusion The development of IgAN may be correlated with IgA precipitation in positive HBVAg kidney tissues with IgAN. The concentration of serum IgA may not be the decisive factor in the occurrence of IgAN.更多还原