【摘要】 目的:研究牙本质基质(DM)对人牙囊细胞(h PFCs)增殖、分化的影响。方法:酶消化组织块法分离培养hDFCs,经鉴定后取第4代细胞并将其随机分为2组,分别用DM浸提液(实验组)和α-DMEM培养液(对照组)进行培养。然后分别用CCK-8法检测各组细胞在培养后1~7 d各时间点的增殖情况;用Western-blot检测培养7 d后各组细胞中ALP、Runx2、OPN、CP-23各成骨/成牙骨质相关基因的蛋白表达水平。结果:DM浸提液对h DFCs的增殖无明显促进作用(P>0.05),但能明显上调其ALP、Runx2、OPN、CP-23蛋白表达水平(P<0.05)。结论:牙本质基质浸提液可诱导hDFCs向成骨/成牙骨质向分化。更多还原

【Abstract】 AIM: To evaluate the effects of dentin matrix( DM) on the proliferation and osteo/cementoblastic differentiation of human dental follicle cells( h DFCs) in vitro. METHODS: Liquid extraction of DM was prepared with DM particles. h DFCs were isolated and cultivated,subsequently cultured in DM extraction. The proliferation of h DFCs was evaluated by CCK-8,and the osteo / cementoblastic differentiation of h DFCs was investigated by detection of ALP,Runx2,OPN,CP-23 using Western blot after 7day co-culture. RESULTS: DM did not show the effect on the proliferation of h DFCs( P > 0. 05),but it increased the expression of ALP,Runx2,OPN and CP-23 after 7d co-culture( P <0.05). CONCLUSION: Dentin matrix can induce the osteo/cementoblastic differentiation of h DFCs.更多还原