【摘要】 GADD45α作为细胞生长阻滞和DNA损伤诱导基因家族成员,参与细胞周期阻滞、细胞凋亡和细胞衰老等调控,在多种因素诱导的细胞应激及DNA损伤应答调控网络中发挥重要生物学功能。多种转录因子和蛋白参与GADD45α基因的转录调控。GADD45α蛋白通过与其他蛋白相互作用在基因组稳定性相关的细胞应答调控中发挥作用。基于GADD45α的细胞调控特性构建的遗传毒性检测系统被应用于外源化合物的体外遗传毒性评价,为遗传毒性评价研究提供了新的思路。本文就GADD45α在遗传毒性检测应用中的分子原理和研究进展进行综述。更多还原

【Abstract】 As a member of growth arrest and DNA damage inducible gene family,GADD45αparticipats in the regulation of cell cycle,cell senescence,cell survival and apoptosis. GADD45α plays a critical role in the responses to cell injury induced by a variety of factors including cell stress and genotoxic chemicals. Different transcription factors and proteins are involved in transcriptional regulation of GADD45α gene. GADD45α protein has been implicated in the regulation of genomic stability related cellular responses through interaction with other proteins. Genotoxicity test systems based on the characteristics of GADD45α in regulation of cell function,can be applied to the detection of potentially genotoxic compounds,which provides new ideas and methods about genotoxicity assessment. The molecular mechanism and research progress of GADD45α in genotoxicity test are summarized in this article.更多还原