【摘要】 目的:探讨基质金属蛋白酶-3(matrix metalloproteinases-3,MMP-3)基因多态位点与承德地区人群2型糖尿病视网膜病变的遗传易感性的关系。方法:应用病例对照研究方法,选取195例糖尿病视网膜病变患者(DR组),其中非增殖性糖尿病视网膜病变(non-proliferative diabetic retinopathy,NPDR)组(n=152)和增殖性糖尿病视网膜病变(proliferative diabetic retinopathy,PDR)组(n=43),205例单纯糖尿病患者(DM组)和261例正常对照组(Control组),采用限制性片段长度多态性(restrictive fragment length polymorphism,RFLP)-聚合酶链反应(polymerase chain reaction,PCR)方法检测MMP-3的基因多态性。结果:MMP-3-1171 5A/6A的等位基因及基因型频率分布在Control组,DM组和DR组之间差异无显著性(P=0.474和P=0.407)。MMP-3-1171 5A/6A的等位基因及基因型频率分布在NPDR和PDR组之间差异无显著性(P=0.724和P=0.820)。结论:MMP-3-1171 5A/6A基因多态性与2型糖尿病视网膜病变的遗传易感性无关。更多还原

【Abstract】 Objective:To determine the correlation between polymorphism at MMP-3 gene and risk of type 2 diabetic retinopathy(DR).Methods:In a hospital-based case-control study,MMP-3 SNP was genotyped by polymerase chain reaction-restrictive fragment length polymorphism(PCR-RFLP) analysis in 195 diabetic retinopathy patients,205 diabetes mellitus patients and 261 healthy persons as control.Results:There is no significant difference between the Control groups,DM group and DR group in allele or genotype distributions of MMP-3-1171 5A/6A(P=0.474 and P=0.407).There is no significant difference between the NPDR and PDR group in allele or genotype distributions of MMP-3-1171 5A/6A(P=0.724 and P=0.820).Conclusion:There is no significant difference between the Control groups,DM group and DRgroup in allele or genotype distributions of MMP-3-11715A/6A.更多还原