【摘要】 目的观察芒果苷、高良姜素对BCBL-1细胞形态及其分泌IL-4的影响。方法将培养的BCBL-1细胞分为不加药物的空白对照组、0.1%二甲基亚砜(DMSO)组、芒果苷组和高良姜素组,观察药物作用24h对细胞形态的影响;收集不同时间段的各处理组的细胞培养上清液,利用ELISA法检测细胞培养上清液中白细胞介素-4(interleukin-4,IL-4)的浓度。结果与相应的对照组相比,BCBL-1细胞培养液中加入药物后,芒果苷组细胞形态无明显变化,细胞培养液中IL-4蛋白含量有上升的趋势(P<0.01);高浓度高良姜素组(25μmol/L)细胞出现细胞皱缩、折光度下降、数量变少,细胞培养液中IL-4蛋白含量在低浓度时有上升的趋势(P<0.05或P<0.01),而在高浓度时有下降的趋势(P<0.01)。结论芒果苷、高良姜素可以调节BCBL-1细胞分泌IL-4。更多还原

【Abstract】 Objective To observe the effects of mangiferin and galangin on BCBL-1cell configurations and its IL-4secretion. Methods The cultured BCBL-1cells were divided into a blank control group without giving drugs,a 0.1% DMSO group,a mangiferin group and a galangin group.Cell morphologic features were observed with microscopy in 24 hours after drug administration.The cell culture supernatants in each administration group at different time were collected and an enzyme-linked immunosorbent assay(ELISA)was performed to detect the concentrations of interleukin-4(IL-4)in cell culture supernatants. Results By comparing with corresponding control group,cells configurations in the mangiferin group had no obvious changes after the BCBL-1cells culture were added with drugs,and the amount of IL-4protein in cell cultures tended to increase(P <0.01).Cells in the high concentration galangin(25μmol/L)group showed wizened,and that the diopter declined and cells amount decreased.The amount of IL-4protein in cells culture by treating with low concentration of galangin tended to increase(P <0.05 or P <0.01),but tended to decrease by treating with high concentration(P <0.01). Conclusion Mangiferin and galangin play a role of regulation of BCBL-1cells in IL-4secretion.更多还原