【摘要】 目的观察紫杉醇诱导的神经病理性大鼠脊髓背角谷氨酸转运体-1(GLT-1)的含量变化。方法雄性SD大鼠45只,随机分成正常对照组(A组),紫杉醇处理组(B组)和紫杉醇溶剂组(C组),A组不作任何处理,B组隔天注射2mg/1 000g体重的紫杉醇,C组隔天注射与B组容积相等的紫杉醇溶剂二甲基亚砜。每天检测大鼠的机械缩足阈值(MWT)。注射的第5d、7d、9d各组均处死5只大鼠,取脊髓背角组织,采用蛋白印迹方法检测各组大鼠GLT-1的表达。结果 B组大鼠的机械缩足阈值在注射第6d时显著降低(P<0.05),并且明显低于A、C组(P<0.05)。A、C两组的MWT值无变化。B组大鼠GLT-1的表达在注射第5d起明显低于A、C两组(P<0.05)。B组大鼠的GLT-1/β-actin的比值与机械缩足阈值呈显著性正相关(r=0.820,P<0.05)。结论紫杉醇诱导的神经病理性疼痛大鼠机械痛敏明显增加,脊髓背角GLT-1表达下调,GLT-1可能参与了紫杉醇致神经病理性疼痛的发生。更多还原

【Abstract】 Objective To investigate the changes of glutamate transporter-1(GLT-1)expression in spinal cord dorsal horns of paclitaxel(Taxol)induced neuropathic pain rats. Methods All the 45 male SD rats inthis study were divided into 3groups randomly:control group(Group A),Taxol group(Group B)and vehicle group(Group C).No administration was given to animals in group A.Animals in group B were injected intraperitoneally with Taxol 2mg/1000 g body weight every other day.Animals in group C were injected intraperitoneally with Taxol solvent DMSO with the same volume as group B at the same time.Mechanical withdrawal thresholds(MWT)of the rats were measured every day.The spinal cord dorsal horn tissues were taken from 5rats in each group on day 5,day 7and day 9after injection respectively.Western bolting was employed to detect the expressions of GLT-1. Results MWTs declined(P <0.05),and compared with group A and group C,MWTs in group B decreased(P <0.05)since day 6after injection,while MWTs showed no significant change in group A or group C.Compared with groups A and C,expression of GLT-1in group B decreased since day 5after injection(P <0.05).The ratio of GLT-1/β-actin showed positive significant correlation with MWT(r =0.820,P <0.05). Conclusion Mechanical hyperalgesia and downregulation of GLT-1expressions were found in paclitaxel induced neuropathic pain rats,which implied that GLT-1may play a role in the pathogenesis of neuropathic pain induced by paclitaxel.更多还原