【摘要】 目的探讨富血小板血浆(PRP)对大鼠腹腔巨噬细胞表型的影响及其可能机制。方法采用含有20%PRP的培养基(实验组)和正常培养基(对照组)体外培养大鼠腹腔巨噬细胞,流式细胞仪检测巨噬细胞表面标记分子CCR7和CD163的表达,ELISA方法检测PRP对上清液中炎症因子IL-4和IL-10表达的影响,Western blot方法检测PRP对巨噬细胞细胞信号传导和转录因子STAT1和STAT6的磷酸化水平的影响。结果与对照组比较,实验组M2型巨噬细胞表面标记分子CD163阳性细胞亚群比例增多,IL-10和IL-4表达增加(P<0.01),磷酸化STAT6蛋白表达上调。结论 PRP能明显促进大鼠腹腔巨噬细胞向M2型转化;其作用机制可能与JAK1/3-STAT6通路被激活有关。更多还原

【Abstract】 Objective To investigate the effect and underlying mechanism of platelet-rich plasma(PRP)on the phenotype of rat peritoneal macrophage.Methods The rat macrophages were cultrued in 20% PRP(group A)or conventioal medium(group B).The phenotype of macrophages was evaluated by testing CCR7 and CD163using flow cytometry.Inflammatory factors of IL-4and IL-10 were assessd by ELISA.Western blot was used to examine the effect of PRP on p-STAT1 and p-STAT6 expressions.Results Compared with group B,the expression of CD163 was significantly upregulated,the expressions of IL-10 and IL-4were significantly higher(P<0.01),and the expression of p-STAT6 was significantly increased in group A.Conclusion PRP is able to promote the conversion of macrophages to M2 macrophage phenotype.The underlying mechanism may be related with the activation of JAK1/3-STAT6 pathway.更多还原