Human umbilical endothelial cells( HUVECs) were fixed by four different fixative,1 cells were fixed with precooled methanolsolution(-20℃); 2 cells were fixed with fresh preparation of 4 g / L paraformaldehyde solution( PFA); 3 cells were fixed with old 4 g / L PFA solution( more than a month); 4 cells were fixed with3. 7% formaldehydesolution. Cells which were fixed with 3. 7% formaldehydesolution maintained fusiform or irregular polygon,and the prostaglandin recepter 4( EP4) immunostaining effect was the b...
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