【摘要】 目的对2008-2013年四川省登革热病例的实验室检测结果进行总结分析。方法收集2008-2013年由华西医院、四川省人民医院等收治的34例登革热住院患者的血清标本,用酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)检测登革病毒的血清Ig M抗体,实时荧光PCR检测病毒核酸,并进行总结分析。结果 34例病例共收集41份血清标本,患者男女比例为3.86∶1,平均年龄37.24岁,病例全年均有。ELISA检测41份标本中31份结果为阳性(阳性率为75.61%);实时荧光PCR检测的21份标本中,8份结果为阳性(阳性率为38.10%)。最后实验室诊断病例为26例。结论不同病程阶段的病例选择不同的方法进行检测,可有效提高诊断登革病毒感染的敏感性。更多还原

【Abstract】 Objective To detect and analyze the Dengue fever cases in Sichuan province in 2008-2013. Methods The serum samples of the 34 patients in West China Hospital and the People’s Hospital of Sichuan Province were collected,and the antibody Ig M in the serum was detected by enzyme- linked immunosorbent assay( ELISA) and the nucleic acids of the Dengue fever virus was detected by real-time PCR. Results In the 34 patients,the sex ratio was 3. 86: 1,and the average age was 37. 24 years old. The disease occurred all the year around. The antibody Ig M was detected from 31 of 41 patients’ sera by ELISA, with a positive rate75. 61%. Twenty patients’ sera were detected by real-time PCR,8 of them were positive,with a positive rate38. 10%. There are 26 confirmed cases. Conclusion At different stages of the disease,the sensitivity of diagnosis of Dengue fever virus infection could be improved by appropriate detection approaches.更多还原