【摘要】 目的建立同时检测抑郁大鼠血浆中文拉法辛及其代谢物O-去甲基文拉法辛的高效液相色谱串联质谱（LC-MS/MS）方法。方法采用甲醇直接沉淀法处理大鼠血浆样品。色谱柱为Agilent Eclipse plus-C18（4.6 mm×100 mm,3.5μm）,流动相为甲醇-水（60∶40,v/v）,流速为0.5 m L·min^-1。正离子多反应监测（MRM）扫描分析,离子通道:文拉法辛m/z 278.15→58.20,O-去甲基文拉法辛m/z 264.10→58.10。结果文拉法辛和O-去甲基文拉法辛的线性范围均为4⁴00 ng·m L^-1,定量下线均为4ng·m L^-1,日内、日间精密度RSD均小于11%,相对提取回收率均在90%¹05%。结论该方法操作简单,灵敏度高,特异性强,可用于抑郁大鼠血浆内文拉法辛和O-去甲基文拉法辛的含量测定。更多还原

【Abstract】 OBJECTIVE To establish a high performance liquid chromatography-tandem mass spectrometry method for the simultaneous determination of venlafaxine and its metabolite O-desmethylvenlafaxine in plasma of depressive rats. METHODS Plasma samples were prepared by direct methanol precipitation. The chromatographic separation was performed on Agilent Eclipse plus C18（4.6mm×100 mm,3.5 μm） column with a mobile phase consisted of methanol-water（ 60 ∶ 40,v / v） at a flow rate of 0. 5 m L·min^-1. The protonated ions of analytes were detected in positive ionization by multiple reaction monitoring mode（ MRM）. The mass transition pairs of m / z 278.15 → 58. 20,m / z 264. 10 → 58. 10 were used to detected venlafaxine and O-desmethylvenlafaxine. RESULTS The linear concentration range for venlafaxine and O-desmethylvenlafaxine were both 4- 400 ng·m L^-1. The limit of quantitation for both was4 ng·m L^-1. Within-batch and between-batch precision RSD were less 11%. The relative extraction recovery was 90%- 105%.CONCLUSION The method has the advantages of simple operation,high sensitivity and specificity,and can be used for the determination studies of venlafaxine and O-desmethylvanlafaxine in depressive rat plasma.更多还原