【摘要】 目的 研究分析人类runt相关转录因子3(RUNX3)蛋白在肝癌组织中的表达变化及其临床意义。方法 采用免疫组化法对84例肝癌组织标本(肝癌组)及40例癌旁组织标本(癌旁组)进行染色观察，比较RUNX3蛋白在2组标本中的表达变化，并分析RUNX3蛋白在肝癌发生、发展中的作用。结果 肝癌组织中RUNX3蛋白阳性表达率高于癌旁肝组织(χ~2=14.849,P<0.05)。Ⅰ+Ⅱ期肝癌患者的RUNX3蛋白阳性表达率高于Ⅲ+Ⅳ期患者(χ~2=5.601,P<0.05);高、中分化患者的RUNX3蛋白阳性表达率为75.56%显著高于低分化患者的17.95%(χ~2=8.697,P<0.05);未发生淋巴结转移患者的RUNX3蛋白阳性表达率高于发生淋巴结转移患者(χ~2=7.571,P<0.05);而不同年龄、性别、肿瘤部位、组织学类型、肿瘤大小、是否存在包膜、是否存在门脉癌栓患者RUNX3蛋白阳性表达率差异无统计学意义(P>0.05)。结论 肝癌组织中RUNX3蛋白表达较癌旁组织下调，并且与肝癌的分期、分化程度、淋巴结转移有关，RUNX3蛋白下调表达与肝癌的发生、发展具有一定的关系。更多还原

【Abstract】 Objective To investigate the level of human runt-related transcription factor 3(RUNX3) protein in hepatocellular carcinoma and its clinical significance. Methods A total of 84 pathologically diagnosed samples of hepatocellular carcinoma(an HCC group) and 40 samples of adjacent tissues(an adjacent group) were selected. Then, immunohistochemical staining was used to observe the levels of RUNX3 in both groups. Meanwhile, the role of RUNX3 in the development of hepatocellular carcinoma was analyzed. Results Among 80 HCC samples were 41(48.81%) positive RUNX3 cases, in contrast with 34(85.0%) positive RUNX3 cases in 40 samples of adjacent tissues, indicating statistical difference between both groups(χ~2=14.849,P<0.05). HCC patients at Stages Ⅰ and Ⅱ produced a positive RUNX3 protein rate of 58.18%, which was significantly higher than 31.03% from patients at Stages Ⅲ+Ⅳ(χ~2=5.601,P<0.05). The positive RUNX3 protein rate was 75.56% in high and medium differentiated patients, which was remarkably higher than 17.95% in patients with low differentiation(χ~2=8.697,P<0.05). The positive RUNX3 protein rate was 56.92% in patients without lymph node metastasis, which was remarkably higher than 21.05% in patients with lymph node metastasis(χ~2=7.571,P<0.05). No significant difference was found between both groups as to age, gender, tumor site, histological type, tumor size, the presence of capsule, the possibility of changes in a positive RUNX3 protein rate for patients with portal vein tumor thrombus(P>0.05). Conclusion The level of RUNX3 protein in HCC tissues is lower than that in the adjacent tissues, which is related to the pathological stage, differentiation degree and lymph node metastasis of HCC. Down-regulation of RUNX3 protein is associated with the development of HCC.更多还原