【摘要】 目的 探讨中期因子(Midkine, MK)基因表达对人乳腺癌MDA-MB-231细胞株裸鼠体内成瘤性的影响。方法 将转染pSilencer-3.1-H1-MK(MK KD组)和pSilencer-3.1-H1-NC(NC组)质粒的乳腺癌细胞分别接种于雌性BALB/c裸鼠右侧前肢腋部皮下，然后于接种第7、14、21、28和35天观察测量肿瘤大小。结果 与NC组相比，MK KD组裸鼠肿瘤形成时间长，并且肿瘤生长速度慢，瘤体重量轻、体积小(P<0.05)。结论 抑制MDA-MB-231乳腺癌细胞MK基因的表达，可以抑制裸鼠体内成瘤能力。更多还原

【Abstract】 Objective To investigate the effects of Midkine(MK) expression on the tumorigenic ability of MDA-MB-231 cells in nude mice. Methods Plasmids pSilencer-3.1-H1-MK(Group MK KD) and pSilencer-3.1-H1-NC(Group NC) were transfected into MDA 231 cells which were then inoculated in the right side of female BALB/c nude mice. The tumor size was measured on Days 7, 14, 21, 28 and 35. Results Compared with Group NC, nude mice in Group MK KD showed longer time to develop tumor, with a slower rate of tumor growth, lower tumor volume and weight(P<0.05). Conclusion The inhibition of MK gene expression in MDA-MB-231 cells can inhibit the growth of tumor in nude mice.更多还原