【摘要】 目的探讨OSAHS患者外周血皮质醇和单个核细胞(PBMC)中11β类固醇羟化脱氢酶(11βHSD)mRNA的表达及其意义。方法采用ELISA法和实时荧光定量PCR(RT-PCR)检测30例中重度男性OSAHS患者和27名正常男性外周血中皮质醇浓度和PBMC中11βHSD mRNA的表达,分析它们与OSAHS患者临床参数间的关系。结果 OSAHS组和对照组外周血皮质醇浓度无显著差异(t=-0.69,P=0.50)。OSAHS组的PBMC中11βHSD1 mRNA相对表达水平较对照组低(t=2.35,P=0.02);11βHSD2 mRNA在2组人群中均无表达。在OSAHS组中并未发现11βHSD1 mRNA的表达与外周血皮质醇、TNF-α和其他临床参数之间存在相关性。结论中重度男性OSAHS患者PBMC中11βHSD1 mRNA的表达水平较对照组低,这种改变的机制目前尚不清楚;但外周血皮质醇浓度较正常人并无明显改变。更多还原

【Abstract】 Objective To investigate the expression and significance of cortisol in the peripheral blood and 11 β-hydroxy steroid dehydrogenase( 11βHSD) mRNA in the peripheral blood mononuclear cell( PBMC) in patients with obstructive sleep apnea hypopnea syndrome( OSAHS). Methods Enzyme-linked immunoadsordent assay( ELISA) and real-time fluorescent quantitative PCR( RT-PCR) were utilized to detect the concentration of cortisol in the peripheral blood and the expression level of 11βHSD mRNA in the PBMC from 30 patients with moderate and severe OSAHS and 27 healthy male individuals. The relationship between the levels of cortisol and 11Βhsd mRNA,and clinical parameters was analyzed. Results The concentration of cortisol in the peripheral blood did not significantly differ between two groups( t =- 0. 69,P = 0. 50). Compared with the control group,the expression level of 11βHSD1 mRNA in the OSAHS group was significantly down-regulated( t = 2. 35,P = 0. 02). However,11βHSD2 mRNA was not expressed in either group. In the OSAHS group,no significant correlation was observed between the expression of 11βHSD1 mRNA and clinical parameters such as peripheral blood cortisol,TNF-α,et al. Conclusions The expression of 11βHSD1 mRNA in the PBMC from male patients with moderate to severe OSAHS was significantly down-regulated compared with that in their healthy counterparts. The underlying mechanism remains unclear. Nevertheless,the concentration of cortisol in OSAHS patients did not significantly differ from that in the healthy controls.更多还原