【摘要】 目的初步分析贵州及周边不同地域种斑蝥体内结合斑蝥素的含量,筛选出抗人肝癌Hep G2细胞效果显著的地域种。方法采用水提结合斑蝥素方式提取有效成分,运用气相色谱-质谱联用技术(GC-MS)分析提取液内的结合斑蝥素含量,采用磺酰罗丹明染色法(SRB法)考察提取液对人肝癌Hep G2细胞的体外抗肿瘤活性,应用倒置相差显微镜检测凋亡细胞的形态学变化。结果贵州省罗甸县境内南方大斑蝥与黄黑小斑蝥体内所含结合斑蝥素含量均高于其他地区同种斑蝥,分别为375、290 mg/L;不同地域种的斑蝥体内结合斑蝥素对人肝癌Hep G2细胞的增殖均有抑制作用,其中贵州茂丼镇境内的2种斑蝥对人肝癌Hep G2细胞的抑制效果明显优于其他地区同种斑蝥,其半数抑制浓度(IC50)分别为0.78、0.65 mg/L;形态学观察结果显示,茂丼镇南方大斑蝥体内结合斑蝥素可促进人肝癌Hep G2细胞凋亡,且凋亡细胞呈现典型的形态特征。结论不同地域不同种斑蝥体内结合斑蝥素的含量存在较大差异;茂丼镇境内2种斑蝥体内结合斑蝥素对人肝癌Hep G2细胞的抑制效果优于其他地域种,可进行深入研究。更多还原

【Abstract】 Objective To preliminarily analyze the in vivo bound cantharidin content derived from the different geo-graphic variants of Mylabrise in Guizhou Province and surrounding regions for screening out the geographic variant with the obvi-ous effect for anti-human hepatocellular carcinoma Hep G2 cells. Methods The water extracting bound cantharidin mode was adopted to extract the effective ingredients;the bound cantharidin content in the extracting solution were analyzed by using GC-MS;the sulforhodamine B(SRB) assay was employed to evaluate the in vitro anti-tumor effect of the extracted liquid on hepatocellular carcinoma Hep G2cells;the morphological alteration of apoptotic cells was detected by phase contrast microscopy. Results The bound cantharidin content of Mylabrisphalerata Pallas and Mylabriscichorii Linnaeus collected in Luodian County of Guizhou Province were higher than that of the same species of Blister beetle collected from other regions,which were 375 mg/L and290 mg/L,respectively;bound cantharidin from different geographical species all had the inhibitory effects on the proliferation of hepatocellular carcinoma Hep G2 cells,in which two kinds of cantharis in the Maojing town of Guizhou Province had the more significantly inhibitory effects on the proliferation of hepatocellular carcinoma Hep G2 cells than that of the same cantharis in other areas,and its median inhibitory conventration(IC50) value was 0.78 mg/L and 0.65 mg/L respectively;the morphological observation results showed that in vivo bound cantharidin of Blister beetle in the Maojing town could promote the apoptosis of human hepato-cellular carcinoma Hep G2 cells,moreover the apoptotic cells appeared typical morphological characteristics. Conclusion The bound cantharidin content has large difference among the different kinds of cantharis collected from different regions. The in-hibitory effects of bound cantharidin from two kinds of cantharis in the Maojing town on the proliferation of hepatocellular carci-noma Hep G2 cells is superior to other geographic species,which may be deeply researched.更多还原