Investigation intracellular trafficking of siRNAs following their delivery to cells is of great interest to elucidate dynamics of siRNA in cytoplasm. In this study, we present a novel confocal laser scanning microscopy(CLSM) method to evaluate a novel delivery system of 3′-peptide-siRNA therapeutic, which was named 3′-p As-siRNA/CLD. This method could not only calculate the content of the intracellular 3′-peptide-siRNA, but also quantify its co-localization with cellular substructure. We observed that 3′-p ...
【基金】
Ministry of Science and Technology of China(Grant No.2012AA022501,2012CB720604);
the National Natural Science Foundation of China(Grant No.20932001,81302626)
【更新日期】
2016-12-27
【分类号】
R91
【正文快照】
1.Introduction
Small interfering RNAs(si RNAs)have been proved to be the main effectors of RNA interference induced in mammalian cells[1].si RNAs can efficiently silence target gene expression in a sequence-specific manner,thus they have been used in di
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