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铁棒锤不定根诱导及培养的初步研究

Preliminary Study on Adventitious Root Induction and Culturing of Aconitum Pendulum Busch

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【作者】 陈红刚杜弢何佳研张鑫杨晓霞

【Author】 CHEN Hong-gang;DU Tao;HE Jia-yan;ZHANG Xin;YANG Xiao-xia;Key Laboratory of Chemistry and Quality for TCM and Tibetan Medicine in Universities of Gansu Province;Institute of Medicinal Plant Genetics and Breeding of Gansu University of Chinese Medicine;

【机构】 甘肃省高校中(藏)药化学质量研究省级重点实验室甘肃中医药大学药用植物遗传育种研究所

【摘要】 目的建立铁棒锤不定根的诱导及增殖培养体系。方法通过组织培养的方法,考察外植体类型及植物生长物质对铁棒锤不定根的诱导及增殖情况的影响。结果不定根诱导能力依次为根>茎>叶,2.5 mg/L吲哚丁酸有利于铁棒锤不定根的诱导及增殖。结论本研究初步建立了铁棒锤不定根的诱导及增殖培养体系,为铁棒锤深层次的资源开发及利用提供了一种新途径。

【Abstract】 Objective To establish the adventitious root induction and culture system of Aconitum pendulum Busch. Methods The influence of different explants and plant growth substances on adventitious induction proliferation of Aconitum pendulum Busch were investigated through tissue culturing. Results Explants induction ability was: root>stem>leaf. 2.5 mg/L IBA was in favor of induction and multiplication adventitious root of Aconitum pendulum Busch. Conclusion The culture system of induction and proliferation adventitious roots of Aconitum pendulum Busch was preliminary established, which provides a new way for resource exploitation and utilization of Aconitum pendulum Busch.

【关键词】 铁棒锤不定根诱导增殖
【Key words】 Aconitum pendulum Buschadventitious rootinductionproliferation
【基金】 甘肃省青年科技基金计划(1308RJYA026);甘肃省高校中(藏)药化学与质量研究省级重点实验室开放基金项目(zzy-2012-03);兰州市科技计划项目(2013-3-115)
  • 【文献出处】 中国中医药信息杂志 ,Chinese Journal of Information on Traditional Chinese Medicine , 编辑部邮箱 ,2016年04期
  • 【分类号】S567.239
  • 【被引频次】5
  • 【下载频次】128
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