In order to improve the properties of bacterial nano-cellulose(BNC) for medical applications, chitosan(CS) was composited with BNC using In Situ biosynthesis of static cultivations, by which CS could be incorporated into BNC nanofiber during its assembly and formed CS/BNC nanofiber composite hydrogels. In this study, the effects of acid type, CS concentration, p H value and autoclaving procedure on dissolution of CS in culture media, as well as on CS/BNC biosynthesis of Gluconacetobacter xylinus, were inves...