【摘要】 目的构建一种整合素cRGD环肽修饰的脂质体(cRGD-LP)并用于靶向A549细胞的研究。方法采用薄膜分散法制备cRGD-LP,测定其粒径和电位,用流式细胞仪考察A549细胞对其的摄取,激光共聚焦显微镜考察cRGD-LP的肿瘤球穿透能力。结果构建的cRGD-LP粒径为(112.3±7.8)nm,粒径分散系数(PDI)为0.023±0.045,电位为(2.38±0.87)m V,且24 h内有良好的血清稳定性,粒径保持在115 nm左右。A549细胞对cRGD-LP的摄取是PEG修饰脂质体(PEG-LP)的1.7倍,且cRGD-LP对A549肿瘤球的穿透能力比PEG-LP高,能穿透至肿瘤球的深部。结论构建的cRGD-LP具有良好的靶向A549细胞的能力,是一种潜在的新型给药系统。更多还原

【Abstract】 Objective To construct a research on liposomes modified by cRGD(cRGD-LP) and its targeting ability to A549 cells in vitro. Methods The cRGD-LP was prepared by film-ultrasonic method. The size and zeta potential of cRGD-LP were detected. The cellular uptake of cRGD-LP to A549 cells was evaluated by flow cytometry. The penetration ability of cRGD-LP into the tumor spheroid was inspected by laser confocal microscopy. Results The size of cRGD-LP was around(112.3 ±7.8)nm; the polydispersity index(PDI) of cRGD-LP was 0.023±0.045; the zeta potential was(2.38±0.87)m V. The cRGD-LP was stable within 24 h and kept the particle diameter at the level of 115 nm. The cellular uptake ability of A549 to cRGD-LP was 1.7-fold as PEG-LP(liposomes modified by PEG). The tumor spheroid penetration ability of cRGD-LP was higher than that of PEG-LP. Conclusion cRGD-LP has high tumor targeting ability to A549 cells, and it is a potential drug delivery system.更多还原