【摘要】 猪δ冠状病毒(PDCo V)是2014年在美国最新检测出的一种猪源冠状病毒。为评估PDCo V在我国的感染和流行情况,根据Gen Bank中登陆的PDCo V M基因核苷酸序列,设计合成1对特异性引物,建立了一种基于M基因的猪δ冠状病毒RT-PCR检测方法。研究结果表明,该方法特异性较好,仅对PDCo V有特异性的扩增,对其它主要猪病病毒核酸扩增均呈阴性;敏感性较高,最低检测限可达6.33×104拷贝/μL。运用该方法对我国华东地区猪场2014~2015年间的492份临床样品进行检测结果显示,PDCo V总阳性率为2.85%(14/492),其中腹泻样品中阳性率为18.60%(8/43),表明本研究建立的RT-PCR方法可以对临床样品进行有效检测。更多还原

【Abstract】 Porcine Deltacoronavirus( PDCo V) is a new swine coronavirus that has been identified in United States since 2014. To investigate the prevalence of PDCo V in China,a RT- PCR assay was developed for detection of PDCo V with a pair of primers designed according to M gene sequence of PDCo V available in Gen Bank. The results showed that the RT- PCR assay was specific for PDCo V detection without cross- reaction with closely related coronavirus or other animal viruses,and was sensitive with the detection limit of approximately 6. 33 × 104 copies / μL. In addition,PDCo V infection was detected in 14 out of 492( 2. 85%) clinical samples collected from pig farms in Eastern Chinafrom 2014 to 2015,and the positive rate was up to 18. 60% in 43 diarrheal samples. It suggested that the PCR assay we developed could be applied as an effective diagnostic technique for detection of PDCoV.更多还原