【摘要】 目的:探讨肿瘤坏死因子TNF-α-308、TNF-α-238位点基因多态性与梅毒发病的关系。方法:采用等位基因特异引物聚合酶链反应(AS-PCR)分析显性梅毒组(A组)共93例;潜伏梅毒组(B组)共55例以及正常对照组(C组)41例TNF-α-308、TNF-α-238位点基因多态性。结果:三组TNF-α-308位点等位基因、基因型频率比较,差异有统计学意义(P<0.05)。三组TNF-α-238位点等位基因频率比较,差异无统计学意义(P>0.05);TNF-α-238位点基因型频率比较,差异有统计学意义(P<0.05)。结论:人梅毒患者中TNF-α-308 A和238A的存在过度表达,且TNF-α-308、238位点基因多态性与梅毒的发病及病程进展可能有关系。更多还原

【Abstract】 Objectives:To investigate the relationship between the polymorphisms of tumor necrosis factor( TNF) 308 &TNF-α-238 and the pathogenesis of syphilis.Methods:Allele-specific polymerase chain reaction( AS-PCR) was used to analyze the promoter allele polymorphism of TNF-α-308 and 238 in Group A( dominant syphilis,93 cases),Group B( recessive syphilis,55 cases) and Group C( control,41 cases).Results:The promoter allele and genotype frequency of TNF-α-308 among the three groups showed statistically significant differences( P < 0.05).The TNF-α-238 promoter allele frequency between the three groups showed no statistically significant differences( P > 0.05),but the TNF-α-238 genotype ~ equency showed statistically significant differences( P < 0.05).Conclusions:Overexpression of TNF-α-308 and 238 A occurs on patients with syphilis.The TNF-α-308,238 A polymorphism may be related with pathogenesis of syphilis.更多还原