【摘要】 为探明黄麻链霉菌NF0919菌株和枯草芽孢杆菌DJ-6对葡萄霜霉病的生防活性,采用叶盘法分别测定黄麻链霉菌NF0919发酵上清液、1.0×10¹¹cfu/g枯草芽孢杆菌DJ-6 WP、代森锰锌和烯酰吗啉对葡萄霜霉病菌的室内毒力,并进行防治葡萄霜霉病的田间试验。结果表明:NF0919菌株发酵上清液、1.0×1011cfu/g枯草芽孢杆菌DJ-6 WP、代森锰锌和烯酰吗啉对葡萄霜霉病菌孢子萌发抑制的EC₅₀值分别为96.2859、86.6038、69.9472、7.2636μg/m L。NF0919菌株发酵20倍液和1.0×10¹¹cfu/g枯草芽孢杆菌DJ-6WP1000倍液对葡萄霜霉病做预防性施药时,2次药后7天的防效分别为71.55%和70.71%,2次药后14天的防效分别为67.54%和68.19%;当做治疗性施药时,2次药后7天的防效分别为59.72%和56.07%,2次药后14天的防效分别为56.88%和57.46%。2种供试生防菌剂的防效相当,且保护效果与50%代森锰锌WP 300倍液均差异不显著,而治疗效果与40%烯酰吗啉SC 200倍液均差异显著。可见,黄麻链霉菌NF0919菌株和枯草芽孢杆菌DJ-6 WP对葡萄霜霉病具有一定的生防潜力与开发的价值。更多还原

【Abstract】 To investigate the biocontrol activity of Streptomyces corchorusii strain NF0919 and Bacillus subtilis DJ-6 WP to grape downy mildew, we determined the indoor toxicity of the supernatant of S. corchorusii strainNF0919, 1.0×10¹¹cfu/g B. subtilis DJ-6 WP, mancozeb and dimethomorph on Plasmopara viticola by using leafdisc method, respectively, and the field control tests were conducted. The results showed that the EC₅₀ values ofthe supernatant of strain NF0919, 1.0 × 10¹¹cfu/g B. subtilis DJ- 6 WP, mancozeb and dimethomorph were 96.2859, 86.6038, 69.9472 and 7.2636 μg/m L, respectively. The values of field control effect in preventiveexperiments on grape downy mildew on the 7th day after 2 times spraying with 20 times liquid of thesupernatant of strain NF0919 and 1000 times liquid of 1.0×10¹¹cfu/g B. subtilis DJ-6 WP were 71.55% and 70.71%, and the values of field control effect on the 14 th day after 2 times spraying were 67.54% and 68.19%,respectively. The values of field control effect in curative experiments on the 7th day after 2 times spraying with 20 times liquid of the supernatant of strain NF0919 and 1000 times liquid of 1.0×10¹¹cfu/g B. subtilis DJ-6 WPwere 59.72% and 56.07%, the values of field control effect on 14 th day after 2 times spraying were 56.88% and57.46%, respectively. The field control effect of the two kinds of the tested biocontrol agents was equivalent.The protective effect between each of the tested biocontrol agents and 300 times liquid of 50% mancozeb WP had no significant difference, but there were significant differences of the efficacy between each of the testedbiocontrol agents and 200 times liquid of 40% dimethomorph SC. In conclusion, the S. corchorusii strainNF0919 and B. subtilis DJ- 6 WP had certain biocontrol potential to grape downy mildew and value ofdevelopment.更多还原