【摘要】 [目的]探讨新城疫病毒(NDV)7793对结肠癌肝转移瘤小鼠肝组织Th1、Th2、Th17类细胞因子表达的影响,为NDV7793作用人结肠癌肝转移的免疫效果研究提供实验依据。[方法 ]取40只健康BALB/c小鼠,随机分为4组(每组10只):PBS组,5-Fu组,NDV7793组,正常对照组。建模及给药结束,采用ELISA法测定肝脏组织上清IL-2、TNF-α、IL-4、IL-10、IL-17的浓度,比较4组间各细胞因子表达差异,分析TNF-α与IL-17表达水平的相关性。[结果]经NDV7793治疗后,结肠癌肝转移瘤小鼠肝组织Th1、Th2、Th17表达均不同程度恢复到正常对照组水平,即Th1类细胞因子IL-2、TNF-α和Th17类细胞因子IL-17表达显著回升,Th2类细胞因子IL-4、IL-10表达显著下降;与5-Fu组相比,NDV7793组的小鼠肝脏各类细胞因子更趋近于正常对照组小鼠的水平;TNF-α表达水平与IL-17表达水平相关性检测,0.749≤r<1,P<0.01,有统计学意义。[结论 ]NDV7793能促进结肠癌肝转移小鼠的肝组织Th1类细胞因子分泌,抑制Th2类细胞因子分泌,使其趋近于正常水平,且小鼠TNF-α与IL-17表达呈正相关。更多还原

【Abstract】 [Objective] To investigate the effect of Newcastle disease virus strain 7793(NDV7793)on the expression of cytokines Th1,Th2 and Th17 in the liver metastasis model of colon cancer in mice,and to provide basic experimental data in the immunotherapy of NDV7793 on liver metastasis model of colon cancer.[Methods] Forty healthy BALB/c mice were randomly divided into 4 groups(n=10 in each group):PBS group,5-Fu group,NDV7793 group and normal control group.After establishing liver metastasis mice model of colon cancer and treatment with different drugs,the expression level of IL-2,TNF-alpha,IL-4,IL-10,and IL-17 in the liver tissues were measured by ELISA method,respectively.The differences in the level of each cytokine were analyzed among the 4 groups and the correlation between TNF-alpha and IL-17 was also analyzed.[Results] The level of these cytokines in liver tissues increased to the normal level after being treated with NDV and 5-Fu.IL-2,TNF-alpha,and IL-17 increased and IL-4 and IL-10 decreased.Compared with 5-Fu treatment group,liver cytokines level in NDV7793 group were more closer to those in the normal control group.The expressions of IL-17 and TNF-alpha were significantly corelated(0.749 ≤r<1,P <0.01).[Conclusion] NDV7793 treatment can effectively promote the production of Th1 type cytokines and inhibit the production of Th2 type cytokines in the liver metastasis mice model from colon cancer,and reverse these cytokines to normal level.It is a positive correlation between the expressions of TNF-alpha and IL-17 in mice.更多还原