OBJECTIVE To develop a more simple, efficient and economical method of total RNA extracted from human non-small cell lung cancer tissues by optimization of traditional TRIzol method, and detect m RNA expression levels of ERCC1, RRM1 and BRCA1. METHODS Thirty-six samples of non-small cell lung cancer(NSCLC, including tumor tissues and normal tissues) were collected and RNA from these samples were extracted through traditional and optimized method, expression levels of ERCC1, RRM1, BRCA1 and housekeeping gene...
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