Gene knockout is an effective approach to investigate gene function of high-molecular-weight glutenin subunit(HMW-GS). In this study, we developed a set of single HMW-GS deletion lines of Ningmai 9(soft wheat) to understand the effects of HMW-GS deletion on kernel quality, quantity of gluten protein fractions and processing quality. Among the 29 lines tested, three lines were wild type and the remaining were knockout mutants including Glu-A1 x, Glu-B1 x, Glu-B1 y, Glu-D1 x, and Glu-D1 y deletion types of fi...
本研究由国家自然科学基金项目(31101146),江苏省农业科技自主创新资金项目(CX(14)2002)和国家现代农业产业技术体系建设专项(CARS-03)资助。This study was supported by the National Natural Science Foundation of China(31101146),the Indigenous Innovation Foundation o