【摘要】 目的检测梅毒血清固定患者外周血中干扰素调节因子4(IRF4)、辅助性T细胞17(Th17细胞)及其相关细胞因子的表达,初步探讨IRF4和Th17在梅毒血清固定形成中的作用。方法梅毒血清固定患者32例,同时以32例健康体检者为对照,分别应用实时荧光定量PCR法检测外周血单一核细胞(PBMC)中IRF4、白细胞介素17(IL-17)和维甲酸相关孤儿受体γt(RORγt)信使核糖核酸(mRNA)的表达;流式细胞术检测Th17与IRF4在CD4+T淋巴细胞中的比例以及IRF4在Th17细胞中的比例。结果梅毒血清固定组PBMC中IRF4mRNA的表达和CD4+IRF4+T细胞的比例,分别为(3.04E-3±5.20E-4);(44.95±2.68)%,与健康对照组的(4.17E-3±2.01E-3)、(46.76±5.13)%相比,差异均无统计学意义(P>0.05)。血清固定组IL-17和RORγt mRNA的表达分别为(3.13E-5±1.18E-5)、(2.21E-4±3.47E-5),与健康对照组的(1.30E-4±4.09E-5)、(5.59E-4±8.39E-5)相比,显著下降,差异有统计学意义(P<0.05);CD4+T淋巴细胞中Th17比例及Th17中IRF4+T细胞比例,血清固定组为(1.35±0.17)%、(50.51±4.06)%,与健康对照组的(1.91±0.19)%、(65.72±4.61)%相比,显著降低,差异具有统计学意义(P<0.05)。结论 Th17可能参与了梅毒血清固定形成的免疫应答。IRF4在梅毒血清固定中可能不发挥直接作用,而是通过作用于Th17而发挥间接作用。更多还原

【Abstract】 Objective To investigate mechanisms of interferon regulatory factor4(IRF4)and Th17 in seroresistance syphilis.Methods 32 patients with seroresistance syphilis were enrolled and 32 healthy people were selected as a control group.The mRNA levels of IRF4,IL-17 and orphan unclear receptor gammat(RORγt)in peripheral blood mononuclear cells(PBMC)were detected by quantitative real-time PCR.The percentage of Th17 cells and IRF4 in CD4+T cells,and the percentage of IRF4 in Th17cells were determined by flow cytometry respectively.Results There was no statistically significant differences of the IRF4 mRNA expression and the percentage of IRF4+cells in CD4+T cells between seroresistance syphilis group(3.04E-3±5.20E-4;44.95% ±2.68%)and health control(4.17E-3±2.01E-3;46.76%±5.13%)(P>0.05).The expression level of IL-17 and RORγt mRNA obviously decreased in seroresistance syphilis(3.13E-5±1.18E-5;2.21E-4±3.47E-5)compared with that in health control(1.30E-4±4.09E-5;5.59E-4±8.39E-5)(P<0.05).The percentage of Th17 cells in CD4+T cells and IRF4+cells in Th17 cells in seroresistance syphilis patients(1.35% ±0.17%;50.51% ±4.06%)were both significantly lower than that of the health control(1.91%±0.19%;65.72%±4.61%)(P<0.05).ConclusionTh17 cells may take part in the pathogenesis of seroresistance syphilis.IRF4 may play an indirect role in seroresistance syphilis through Th17 cells.更多还原