【摘要】 目的了解上海市2007-2013年艾滋病病毒（HIV）-1亚型流行趋势。方法选取2007-2013年确认的、首次随访的HIV-l感染者1296例。取其血浆标本,应用反转录-聚合酶链反应（RT-PCR）扩增,DNA测序,系统进化树分析,并结合流行病学资料进行分析。结果获得1085例pol区基因片段,其中亚型分布显示,CRF01_AE所占比例为59.1%（641例）,CRF07_BC为23.1%（251例）,B亚型为9.3%（101例）,CRF08_BC为4.2%（46例）,C亚型为1.1%（12例）,CRF55₀1B为0.9%（10例）,CRF59₀1B为0.8%（9例）,CRF02_AG为0.6%（6例）,CRF58₀1B为0.3%（3例）,未定型CRF₀1B为0.3%（3例）,CRF67₀1B为0.2%（2例）和G亚型为0.1%（1例）。HIV-1主要流行毒株,CRF01_AE的比例从2007年64.3%下降到2013年的55.2%,CRF07_BC从2007年的8.6%上升到2013年的30.2%。CRF01_AE、CRF07_BC和B亚型均以性传播途径为主,其中同性性传播分别为61.3%（393/641）、49.8%（125/251）、51.5%（52/101）,异性性传播分别为36.3%（233/641）、35.5%（89/251）、44.6%（45/101）;CRF08_BC中异性性传播占60.9%（28/46）;C亚型、CRF55₀1B、CRF59₀1B和CRF58₀1B均以性传播为主;CRF02_AG以异性性传播为主,未定型CRF01_B、CRF67₀1B和G亚型均以同性性传播为主。结论上海市2007-2013年HIV-1亚型变化明显,目前以CRF01_AE和CRF07_BC为主。继续加强对HIV-1基因亚型监测具有重要意义。更多还原

【Abstract】 Objective To better understand the human immunodeficiency virus-1（HIV-1）phylogenetical tendency from 2007 to 2013in Shanghai.Methods A total of 1296 people with HIV-1-infection living in Shanghai,were randomly sampled and their plasma was collected.The samples were amplified by nest RT-PCR,DNA was sequenced and phylogenetically analyzed.All results were analyzed in combination with the data of epidemiological survey.Results With fragments of 1085pol-gene gathered,phylogenetical tendency showed that CRF01_AE was predominant by 59.1%（641）,and followed by CRF07_BC of 23.1%（251）,subtype B of 9.3%（101）,CRF08_BC of4.2%（46/1985）and subtype C of 1.1%（12）,CRF55₀1of 0.9%（10）,CRF59₀1Bof 0.8%（9）,CRF02_AG of 0.6%（6）,CRF58₀1Bof 0.3%（3）,no subtypeCRF₀1Bof 0.3%（3）,CRF67₀1Bof 0.2%（2）,and subtype G of0.1%（1）respectively.CRF01_AE was decreased from 64.3% in 2007 to 55.2%in 2013,while CRF07_BC increased from 8.5%in 2007 to 30.2%in2013.CRF01_AE,CRF07_B and subtype B were spread by sexual transmission,with homosexuals by61.3%（393/641）,49.8%（125/251）,51.5%（52/101）respectively,and heterosexuals by 36.3%（233/641）,35.5%（89/251）,44.6%（45/101）;while CRF08_BC was mainly spread by heterosexual transmission,by 60.9%（28/46）,Subtype C,CRF55₀1B,CRF59₀1Band CRF58₀1Bwere all spread by sexual transmission.CRF02_AGwas spread by heterosexual transmission.Not stereotyped CRF01_B,CRF67₀1Band subtype G were all spread by homosexual transmission.Conclusion HIV-1phylogenetical change is apparent in Shanghai,and currently the priority subtypes are CRF01_AE and CRF07_BC.Continuing surveillance on HIV-1subtype gene-types is of great importance.更多还原