The enzymatic saccharification parameters of cotton stalk pretreated by ethylene glycol were investigated.The effect of hydrolysis time,hydrolysis temperature,pH,substrate mass concentration,and cellulase concentration on reducing sugar mass fraction was studied by the single factor experiment and Box-Behnken design to optimize enzymatic saccharification conditions.The results indicated that the influence of cellulase concentration and hydrolysis time on reducing sugar mass fraction was highly significant,a...