【摘要】 目的探讨白细胞介素22(IL-22)对SD新生大鼠高氧肺损伤的保护作用。方法新生SD大鼠随机分为对照组、高氧组和IL-22(10 ng/g)治疗组,每组随机分成1、3、7 d三个亚组。检测各组大鼠体质量、HE染色检测肺组织病变、实时定量PCR检测肺组织肿瘤坏死因子α(TNF-α)mRNA、ELISA检测血浆IL-1β水平。结果高氧暴露1 d,三组大鼠体质量差异不显著,肺组织病理未见异常。高氧后3、7 d,高氧组体质量明显低于对照组和治疗组,血浆IL-1β水平显著增高,肺组织结构破坏,7 d损伤更显著;高氧组肺组织TNF-αmRNA水平增高,IL-22治疗后,肺组织TNF-αmRNA水平明显降低。结论 IL-22对高氧诱导的肺损伤有保护作用。更多还原

【Abstract】 Objective To study the protective effect of interleukin-22( IL-22) against the hyperoxia-induced lung injury in neonatal SD rats. Methods The neonatal SD rats were randomized into control group,hyperoxia group and IL-22( 10 ng / g)treatment group. Each group was randomly divided into three subgroups of 1,3,7 days( n = 9). Body mass in every group was detected; lung pathological changes were observed by HE staining; tumor necrosis factor α( TNF-α) in lung tissues was tested by quantitative real-time PCR; and IL-1β in plasma was measured by ELISA. Results After exposure to hyperoxia for1 day,the body mass in the three groups showed no significant difference,and the structure of lung tissues were normal.After exposure to hyperoxia for 3 or 7 days,the body mass in the hyperoxia group was significantly lower,IL-1β in plasma was significantly enhanced,and the structure of lung tissues were destroyed,which aggravated with the time of hyperoxia exposure. TNF-α mRNA level increased obviously in the hyperoxia group. However,when treated with IL-22,TNF-α mRNA was significantly down-regulated in the treatment group. Conclusion IL-22 may play an protective role in hyperoxia-induced lung injury.更多还原