【摘要】 目的研究槐叶萍[Salvinia natans(L.)All.]养殖水的抑藻效应及抑制机制。方法将不同浓度5%、10%、20%和40%槐叶萍养殖水作用于7.5×105cells/m L的铜绿微囊藻(Microcystis aeruginosa),测定其对藻细胞的溶藻率、电导率、藻液核酸和蛋白含量、丙二醛(MDA)含量以及超氧化物歧化酶(SOD)活性等的影响。结果40%处理组第4 d的抑制率达100%,5%处理组第7 d的抑制率达65%,藻细胞溶藻率、电导率、藻液中核酸含量,蛋白含量,以及藻细胞MDA含量均随养殖水浓度的增大而升高,SOD酶活性随着槐叶萍养殖水浓度增大先上升,然后又下降。结论槐叶萍养殖水通过改变藻细胞结构和胞内酶活性等抑制铜绿微囊藻的生长。更多还原

【Abstract】 Objective To study the inhibiting effect of aquaculture water of Salvinia natans( L.) All. on Microcystis aeruginosa and the mechanism. Methods Microcystis aeruginosa of 7. 5 × 105 cells / m L was treated by the culture water of Salvinia natans density of 5%,10%,20% and 40%,respectively. The inhibition ratios were calculated,conductivity of algal cells,content of nucleic acid and protein in algae solution,influence of superoxide dismutase( SOD) activity and malondialdehyde( MDA) content were measured. Results The inhibition rate of 40% treatment group up to 100% on the fourth day. The inhibition rate of 5% treatment group up to 65% on the 7th day. The conductivity of algae cell gone up while the concentration of cultured water was increasing,as well as its content of nucleic acid,protein and MDA. However,the activity of SOD rose at first and then fall down while the concentration of cultured water of Salvinia natans was increasing. Conclusion The aquaculture water of Salvinia natans has a powerful inhibitory effect on Microcystis aeruginosa by changing the structure of algal cells and intracellular enzyme activity,etc.更多还原