Objective Objective To detect the residual DNA quantity in Vero cell contained in rabies vaccine( Vero cell)for human use( simplified as rabies vaccine) by Digoxigenin-labeled DNA probe,and the applicability of the method was verified and applied. Methods The Digoxigenin-labeled DNA probe was carried out in verification of the specificity,sensitivity,repeatability and stability for detecting the residual DNA quantity in Vero cell contained in rabies vaccine,and the method was applied to detect residual DNA ...
【更新日期】
2016-04-19
【分类号】
R392-33
【正文快照】
Vero细胞是世界卫生组织(World Health Or-ganization,WHO)推荐使用制备人用疫苗的培养基质,该传代细胞系,生长快、易培养,并在规模化病毒培养(生物反应器)中显现出极大的优势,解决了病毒性疫苗规模化生产的瓶颈。Vero细胞DNA具有潜在的致瘤性[1-2],因此,以Vero细胞为基质的疫?