【摘要】 目的分析乙肝患者的血清标志物和乙肝病毒(HBV)DNA联合检测在HBV感染诊断中的应用价值。方法以2013年6月-2015年6月在珠海市妇幼保健院就诊的120例乙肝患者血清样本作为研究资料,分别采用ELISA定性试验和实时荧光定量PCR(RT-PCR)检测HBV血清标志物(HBs Ag、抗-HBs、HBe Ag、抗-HBe、抗-HBc)和HBV DNA含量。结果Ⅰ组HBV DNA阳性率为96.15%;Ⅱ组HBV DNA阳性率为48.08%;Ⅲ组HBV DNA阳性率为4.76%。Ⅰ组HBV DNA定量显著高于Ⅱ、Ⅲ两组(P<0.01);Ⅱ组HBV DNA定量显著高于Ⅲ组(P<0.05)。HBV DNA与HBs Ag检出符合率分别为73.33%和72.50%,两者之间差异无统计学意义(P>0.05)。结论乙肝血清标志物定量与HBV DNA定量联合检测在诊断HBV感染过程中起互相补充的作用,诊断结果更加准确,可提供更有效的参考依据。更多还原

【Abstract】 Objective To analyze the application value of combination detection in the diagnosis of hepatitis B virus(HBV) infection by the quantitative detection of serological markers and HBV DNA of hepatitis B patients. Methods The serum samples of 120 hepatitis B patients from Jun. 2013 to Jun. 2015 were collected,and the ELISA qualitative test and RT-PCR were used to detect the contents of HBV serological markers( HBs Ag,anti-HBs,HBe Ag,anti-HBe,anti-HBc) and HBV DNA. Results The HBV DNA positive rate was 96. 15% in Ⅰ group; the HBV DNA positive rate was 48. 08% in Ⅱ group; the HBV DNA positive rate was 4. 76% in Ⅲ group. The quantitative HBV DNA in Ⅰgroup was significantly higher than that in Ⅱ and Ⅲ groups( P < 0. 01); the quantitative HBV DNA in Ⅱ group was significantly higher than that in Ⅲ group( P < 0. 05). The detection coincidence rates of HBV DNA and HBs Ag were73. 33% and 72. 50%,respectively,and there was no significant difference between them( P > 0. 05). Conclusion The combination detection of HBV serological markers and HBV DNA quantification plays a complementary role in the diagnosis of HBV infection,and has a more accurate diagnosis as well as can provide a more effective reference.更多还原