【摘要】 利用2-氟[18F-2]脱氧葡萄糖([18 F]fluorodeoxyglucose,18F-FDG)乳腺癌细胞摄取实验筛选高糖酵解率的乳腺癌细胞;采用基因沉默技术,降低细胞烟酰胺腺嘌呤二核苷酸磷酸氧化酶4(NOX 4)表达;采用免疫印迹方法检测三组细胞(空白对照组、非特异性siRNA转染组和特异性NOX 4-siRNA转染组)的上皮间质转化(epithelial mesenchymal transition,EMT)相关分子HIF-1α和TGF-β表达水平;通过细胞侵袭实验分析三组细胞的侵袭能力差异。结果显示,高侵袭力的MDA-MB-231较低侵袭力的MCF-7乳腺癌细胞系具有高糖酵解率(t=10.52,P<0.05);抑制MDA-MB-231细胞的NOX 4表达,降低了细胞EMT相关因子HIF-1α、TGF-β表达(P均<0.01)与细胞侵袭能力(t=-8.0,P<0.01)。本研究初步证实NOX 4失活能够降低高糖代谢乳腺癌细胞的侵袭转移能力。更多还原

【Abstract】 The breast cancer cells with high glucose metabolism were screened by 18 FFDG cell uptake experiment.The gene silencing technique was used to reduce NOX 4expression in cells.The experiment was divided into three groups:control group,the non-specific transfection group and specific transfection group.Western blotting was used to detect differences in the expression of HIF-1αand TGF-βin three groups.The invasion ability of the three groups by transwell cell invasion assay was analyzed.The results showed that MDA-MB-231 breast cancer cells had a higher rate of glycolysis than MCF-7tumor cells(t=10.52,P<0.05).The expression of HIF-1αand TGF-βand the invasiveness of cells were reduced by inhibiting the level of NOX 4.This study showed that inhibition of NOX 4expression was able to inhibit the invasion of breast cancer cell with high glucose metabolism.更多还原