【摘要】 为探究¹²⁵I标记重组抗蓖麻毒素（Ricin）人源化单克隆抗体(¹²⁵I-MIL50)在大鼠体内的药代动力学、生物分布及排泄特点,采用Iodogen法对MIL50进行¹²⁵I标记,结合TCA法测定不同时间¹²⁵IMIL50在大鼠血清、组织、体液和排泄物中的含量。结果表明:Ricin染毒组和直接给药组血药浓度在14d内没有差异,14d后染毒组¹²⁵I-MIL50消除快于未染毒组;血清中¹²⁵I-MIL50浓度高于其他组织和体液,肾、膀胱组织中浓度较高,脑、脊髓、脂肪等脂性组织中浓度一直较低;¹²⁵I-MIL50给药后27d内经尿累积排泄率约为62.6%,经粪便的排泄率为15.5%。研究结果为临床实验和给药方案提供参考依据。更多还原

【Abstract】 In order to study the impact of Ricin on the pharmacokinetics of ¹²⁵I-MIL50 and to investigate the tissue distribution and excretion of ¹²⁵I-MIL50 in Wistar rats,MIL50 was labeled with 125 I using the Iodogen method.Then,the concentration of ¹²⁵IMIL50 in serum、tissues、body fluids and excretions was measured by TCA method at different time.The results showed that 25I-MIL50 was eliminated faster after 14 days in the Ricin administrated group.The concentration of ¹²⁵I-MIL50 in serum was always higher than that in other tissues.The level in kidney and bladder were high and in brain,spine and fat were low.The cumulative excretion rate of ¹²⁵I-MIL50 was 62.6%in urine,and 15.5%in feces within 27 days.Ricin could fasten the elimination of ¹²⁵I-MIL50 when the concentration of ¹²⁵I-MIL50 was low in Wistar rats.It might because of the interaction between antigen and antibody.¹²⁵I-MIL50 had no specific combination with tissues and it could hardly entered into lipophilic tissues.Urinary excretion represented the major pathway of elimination of ¹²⁵I-MIL50.The results of the study provide a reference for clinical trials and drug administration program.更多还原