【摘要】 目的研究人肺腺癌A549细胞放射治疗组与对照组的胰岛素受体(IR)的表达,探讨胰岛素受体与放射敏感性的关系。方法将随机分为对照组(0 Gy)和放射治疗组(剂量为2 Gy、4 Gy、6 Gy、8 Gy、10 Gy、12 Gy 6 MV X线)人肺腺癌A549细胞进行放射治疗,放疗后培养25天后用免疫组化S-P法测定肺腺癌A549细胞株各标本的胰岛素受体表达情况。结果胰岛素受体(IR)主要分布于A549细胞膜上。小剂量(2 Gy、4 Gy)组与对照组(0Gy)胰岛素受体表达强阳性率差异无统计学意义。6 Gy、8 Gy、10 Gy、12 Gy放射治疗组与对照组相比,胰岛素受体强阳性率与对照组差异有统计学意义(P<0.005),其中12 Gy剂量组强阳性率最高。结论胰岛素受体与放射敏感性负相关,ISR强阳性的肿瘤细胞对射线抗拒。胰岛素受体有可能成为预测肺癌放射敏感性的分子标志物。更多还原

【Abstract】 Objective: To find relation between insulin receptorand cancer radiosensitivity. Methods: A549 cells were randomly divided into two groups,matched group( 0 Gy) and radiation groups( 2 Gy,4 Gy,6 Gy,8 Gy,10 Gy,12 Gy with 6MV X rays). The cells were radiated and the cells were cultured for 25 days after radiotherapy and expression of lung adenocarcinoma A549 cell line samples were detected by Immunohistochemistry( SP method). Results: Insulin receptor was located mainly in the A549 cell membrane. There was no difference in the expression of insulin receptorbetween small dose( 2 GY,4 GY) groups and control group( 0 GY). The groups with the doses of 6 GY,8 GY,10 GY and 12 GY were compared with the control group,and the insulin receptor expression had statistical significance( P < 0. 005),and the highest dose group with dose of 12 GY had strongest positive expression. Conclusion: Insulin receptor and radiosensitivity have negative correlation,and tumor cells with ISR strong positive are resistant to radiation. Insulin receptor may become one of cancer radiosensitivity predictive molecular markers.更多还原