【摘要】 研究了处理后白花丹参药材中丹酚酸A的超声波提取最佳工艺条件。采用超声波提取方法,以HPLC测定药材中丹酚酸A的提取率为指标,通过单因素实验考察乙醇浓度对丹酚酸A提取率的影响,在此基础上,通过L9(34)正交实验考察料液比、提取时间、提取温度、提取次数对丹酚酸A提取率的影响。结果表明最佳提取工艺条件:以料液比为1∶12的75%乙醇在30℃条件下超声波提取40 min,共提取3次。在此条件下测得处理白花丹参药材中丹酚酸A的平均含量为17.1810 mg/g。该方法稳定性和重复性好,为丹酚酸A的工业化生产提供了参考。采用THP-1巨噬细胞对得到的丹酚酸A提取物进行了抗炎活性评价,结果显示该提取物具有较好的抗炎活性。更多还原

【Abstract】 In this study,the ultrasonic extraction technology of salvianolic acid A( Sal A) from the treated roots of Salvia miltiorrhiza Bunger var. alba Wu and Li,mss. was optimized,and the content of Sal A,as the marker component for the process,was measured by HPLC. The effect of ethanol concentration on the extraction efficiency of Sal A from the treated materials was studied by single factor experiment. Then,the effects of solid-liquid ratio,extraction duration,temperature,and times of extraction on the extraction efficiency of Sal A were investigated using L9( 34) orthogonal experiment. The optimal extraction conditions were as follows: the materials were extracted under ultrasonication in 75% ethanol at 30 ℃for 3 times,with solid-liquid ratio 1∶ 12 and each time for 40 minutes. The extraction process was validated to be stable and repeatable,and it provided a method for the industrial extraction of Sal A from the treated materials. In addition,the extracts of Sal A were evaluated for the anti-inflammation activity with THP-1cells and the results showed that they had moderate anti-inflammatory activity.更多还原